Bio-Adembeads PAG - Immunoprecipitation
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PAG = Protein AG is a genetically-engineered protein that combines the IgG binding domains of both Protein A and G.

Recombinant fusion protein AG contains five Ig-binding regions of protein A and three Ig-binding. Protein AG binds to all IgG subclasses from various mammalian species, including all IgGs that bind both Protein A and Protein G, making the ideal choice for purification of all kinds of polyclonal and monoclonal IgG antibodies. The protein AG lacks albumin, cell wall binding regions and others non-specific binding regions to ensure maximum specific IgG binding.


 
  • High specificity- Less background
  • Bind all species of IgGs
  • Cross-linking procedure: allow not to release the antibody during elution step
  • PAG Elution Buffer (available separately): optimized elution method in order not to release antibody crosslinked to Bio-Adembeads PAG
    Simplify  and speed the procedure
  • No centrifugation
  • Less incubation time
  • Washing step are reduced
  • No preclearing step required
  • Clear visualization of the magnetic particles
  • Gentle system:
    • Minimal loss of protein
    • Minimal stress versus centrifugation
    • Isolation of large protein complex
    • Flexible: binds to a wider range of antibodies
    • Recombinant form of the protein AG without albumin-binding sites, thus reducing co-purification of contaminating proteins. Only the Fc binding sites are present.

     

    Bio-Adembeads PAG (300nm) coated with recombinant high quality Protein AG covalently coupled to the surface. This product is available in:
    - 2ml /04630 (100 immunoprecipitations)
    - 5ml / 04631 (250 immunoprecipitations)
    Supplied in storage buffer (code number 10201) proclin 300 as a preservative

    PAG Elution Buffer (10701), 10ml.

    References for Bio-Adembeads Protein A/G/AG and ChIP-Adembeads Protein A/G
    1- Genes & Development 20:836-847, 2006, Role of heat-shock factor 2 in cerebral cortex formation and as a regulatorof p35 expression, Yunhua Chang, Päivi Östling, Malin Åkerfelt, Diane Trouillet, Murielle Rallu, Yorick Gitton, Rachid El Fatimy, Vivienne Fardeau, Stéphane Le Cro, Michel Morange, Lea Sistonen and Valérie Mezger
     
    2-  Nucleic Acids Research Advance Access published online on April 25, 2007, A bona fide La protein is required for embryogenesis in Arabidopsis thaliana, Sophie Fleurdépine, Jean-Marc Deragon, Martine Devic, Jocelyne Guilleminot and Cécile Bousquet-Antonelli

    3- The Plant Journal Volume 50 Issue 2 Page 197-206, April 2007, The auxin-binding protein 1 is essential for the control of cell cycle Karine M. David, D. Couch, N. Braun, S. Brown, J. Grosclaude and C. Perrot-Rechenmann

    4- Journal of Cell Communication and Signaling, 1873-9601, 8 September 2007 Domain-specific CCN3 antibodies as unique tools for structural and functional studies, Noureddine Lazar, Cristina Manara, Samuel Navarro, Anne-Marie Bleau, Antonio Llombart-Bosch, Katia Scotlandi, Nathalie Planque and Bernard Perbal

    5- Talanta (2008):Enzymes-linked immunosorbent assay ELISA based on superparamagnetic nanoparticles for aflatoxin M1 detection, A.Radoi, M targa, B.Prieto-Simon, J l Marty
     
    6- Journal of Bacteriology, August 2009, p. 4943-4950, Vol. 191, No. 15,The StkP/PhpP Signaling Couple in Streptococcus pneumoniae: Cellular Organization and Physiological Characterization, Makoto Osaki,Tania Arcondéguy, Amandine Bastide, Christian Touriol, Hervé Prats, and Marie-Claude Trombe

    7- Blood, 2009 Sep 17;114(12):2427-38. Epub 2009 Jul 14, Human T-cell leukemia virus type 2 produces a spliced antisense transcript encoding a protein that lacks a classic bZIP domain but still inhibits Tax2-mediated transcription, Halin M, Douceron E, Clerc I, Journo C, Ko NL, Landry S, Murphy EL, Gessain A, Lemasson I, Mesnard JM, Barbeau B, Mahieux R.

    8- PLoS Pathog., 2009 Jul;5(7):e1000521. Epub 2009 Jul 17, NRP/Optineurin Cooperates with TAX1BP1 to potentiate the activation of NF-kappaB by human T-lymphotropic virus type 1 tax protein. Journo C, Filipe J, About F, Chevalier SA, Afonso PV, Brady JN, Flynn D, Tangy F, Israël A, Vidalain PO, Mahieux R, Weil R.

    9- Brain Research Bulletin, Volume 80, Issues 4-5, 28 October 2009, Pages 296-301,The presenilin-1 familial Alzheimer's disease mutation P117L decreases neuronal differentiation of embryonic murine neural progenitor cells, Lorenza Eder-Colli, Noelia Abad-Estarlich, Carine Pannetier, Philippe G. Vallet, Claude Walzer, Gregory A. Elder, Nikolaos K. Robakis, Constantin Bouras, Armand Savioz

    10- Human Pathology, Volume 40, Issue 10, October 2009, Pages 1479-1486,  Prognostic relevance of CCN3 in Ewing sarcoma, Bernard Perbal, Noureddine Lazar, Diana Zambelli, Jose Antonio Lopez-Guerrero, Antonio Lombart-Bosch, Katia Scotlandi, Piero Picci 

    11- Genes & Dev. 2009. 23: 1399-1407, Metabolic intermediates selectively stimulate transcription factor interaction and modulate phosphate and purine pathways Benoît Pinson, Sabine Vaur,Isabelle Sagot, Fanny Coulpier, Sophie Lemoine, Bertrand Daignan-Fornier 


     
     
     
     Bio-Adembeads Protein A
     Bio-Adembeads Protein G
     PAG Elution Buffer
     
     Adem-Mag MODULO
     
     Protocol
     Brochure
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