ChIP-Adem-Kit Protein A
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The ChIP-Adem-Kit is an innovative system especially designed for monitoring transcription factors or histones / DNA interactions. The ChIP-Adembeads Protein A/G after incubation with the Blocking Buffer have a specific surface designed for efficient Protein / DNA complex immunoprecipitation and to minimize non-specific enrichment. Proteins and other contaminants are eliminated in the washing steps. The ChIP protocol is greatly simplified by using blocked Protein A/G-coated nanoparticles. Some steps have been completely eliminated. Unlike traditional agarose beads, chromatin pre-clearing is not required. ChIP-Adembeads combined to designed buffers reduce background and maximize the amount of templates available for the reaction, achieving greater sensitivity where maximum DNA recovery is critical. The isolated DNA can be used in downstream applications : PCR and Real-Time PCR.
 
  • Higher efficiency
    • Effective IP and low non specific binding due to optimized magnetic nanoparticles
    • ChIP-Adembeads Protein A/G after incubation with Blocking buffer have a specific surface designed for efficient Protein DNA complex immunoprecipitation and to minimize non-specific enrichment
    • ChIP-Adembeads combined to Blocking Buffer reduce background
    • No sedimentation problem
  • Save time and effort
    • Spin steps have been replaced by rapid magnetic separations, reducing the amount of hands-on time during the assay
    • Time consuming steps are reduced or eliminated
    • No pre-clearing step
  • Easy to use: ChIP-adem-Kit contains all necessary components for successful monitoring of transcriptions factors and histones / DNA interactions
  • Enhance sensitivity: method more effective compare to sepharose beads
  • Ideal for many samples processing


 

Product                                           Size                                                                  Code Number

ChIP-Adembeads Protein A               Protein A beads + Blocking Buffer, 40 ChIP          04240
                                                       

ChIP-Adem-Kit Protein A                   Complet kit, 25 ChIP                                         04241

References for Bio-Adembeads Protein A/G/AG and ChIP-Adembeads Protein A/G
1- Genes & Development 20:836-847, 2006, Role of heat-shock factor 2 in cerebral cortex formation and as a regulatorof p35 expression, Yunhua Chang, Päivi Östling, Malin Åkerfelt, Diane Trouillet, Murielle Rallu, Yorick Gitton, Rachid El Fatimy, Vivienne Fardeau, Stéphane Le Cro, Michel Morange, Lea Sistonen and Valérie Mezger
 
2-  Nucleic Acids Research Advance Access published online on April 25, 2007, A bona fide La protein is required for embryogenesis in Arabidopsis thaliana, Sophie Fleurdépine, Jean-Marc Deragon, Martine Devic, Jocelyne Guilleminot and Cécile Bousquet-Antonelli

3- The Plant Journal Volume 50 Issue 2 Page 197-206, April 2007, The auxin-binding protein 1 is essential for the control of cell cycle Karine M. David, D. Couch, N. Braun, S. Brown, J. Grosclaude and C. Perrot-Rechenmann

4- Journal of Cell Communication and Signaling, 1873-9601, 8 September 2007 Domain-specific CCN3 antibodies as unique tools for structural and functional studies, Noureddine Lazar, Cristina Manara, Samuel Navarro, Anne-Marie Bleau, Antonio Llombart-Bosch, Katia Scotlandi, Nathalie Planque and Bernard Perbal

5- Talanta (2008):Enzymes-linked immunosorbent assay ELISA based on superparamagnetic nanoparticles for aflatoxin M1 detection, A.Radoi, M targa, B.Prieto-Simon, J l Marty
 
6- Journal of Bacteriology, August 2009, p. 4943-4950, Vol. 191, No. 15,The StkP/PhpP Signaling Couple in Streptococcus pneumoniae: Cellular Organization and Physiological Characterization, Makoto Osaki,Tania Arcondéguy, Amandine Bastide, Christian Touriol, Hervé Prats, and Marie-Claude Trombe

7- Blood, 2009 Sep 17;114(12):2427-38. Epub 2009 Jul 14, Human T-cell leukemia virus type 2 produces a spliced antisense transcript encoding a protein that lacks a classic bZIP domain but still inhibits Tax2-mediated transcription, Halin M, Douceron E, Clerc I, Journo C, Ko NL, Landry S, Murphy EL, Gessain A, Lemasson I, Mesnard JM, Barbeau B, Mahieux R.

8- PLoS Pathog., 2009 Jul;5(7):e1000521. Epub 2009 Jul 17, NRP/Optineurin Cooperates with TAX1BP1 to potentiate the activation of NF-kappaB by human T-lymphotropic virus type 1 tax protein. Journo C, Filipe J, About F, Chevalier SA, Afonso PV, Brady JN, Flynn D, Tangy F, Israël A, Vidalain PO, Mahieux R, Weil R.

9- Brain Research Bulletin, Volume 80, Issues 4-5, 28 October 2009, Pages 296-301,The presenilin-1 familial Alzheimer's disease mutation P117L decreases neuronal differentiation of embryonic murine neural progenitor cells, Lorenza Eder-Colli, Noelia Abad-Estarlich, Carine Pannetier, Philippe G. Vallet, Claude Walzer, Gregory A. Elder, Nikolaos K. Robakis, Constantin Bouras, Armand Savioz

10- Human Pathology, Volume 40, Issue 10, October 2009, Pages 1479-1486,  Prognostic relevance of CCN3 in Ewing sarcoma, Bernard Perbal, Noureddine Lazar, Diana Zambelli, Jose Antonio Lopez-Guerrero, Antonio Lombart-Bosch, Katia Scotlandi, Piero Picci 

11- Genes & Dev. 2009. 23: 1399-1407, Metabolic intermediates selectively stimulate transcription factor interaction and modulate phosphate and purine pathways Benoît Pinson, Sabine Vaur,Isabelle Sagot, Fanny Coulpier, Sophie Lemoine, Bertrand Daignan-Fornier 

 


 
 
 
 Bio-Adembeads Protein A for immunoprecipitation
 ChIP-Adem-Kit Protein G
 
 Adem-Mag SV
 Adem-Mag MODULO
 
 Protocol
 Brochure
 Quick Protocol (english)
 Quick protocol (french)
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