1-Ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC) is a water-soluble derivative of carbodiimide. Carbodiimide catalyzes the formation of amide bonds between carboxylic acids or phosphates and amines by activating carboxyl or phosphate to form an O-urea derivative. This derivative reacts readily with nucleophiles (see figure below). The reagent can be used to peptide bonds from acid and amines. |