Ig Adem Kit is designed for simple and rapid immunoglobulin purification from serum, ascites culture or hybridoma supernatants. The kit was developed to overcome the drawbacks of the commonly used purification methods. Obtaining pure antibodies by removing non relevant protein often present in high abundance is a critical step. Immunoglobulins purification is based on the ability of some proteins to bind to a ligand that contains a sulfone group close to a thioether group. This binding event is termed thiophilic adsorption. Salts that interact with water molecules, such as potassium and ammonium sulfate, promote this binding. Magnetic beads bind IgG (subclasses), IgA, and IgM in the same proportion, present in starting material. The purified Ig can be used in a variety of applications including: ELISA, Western Blotting, Immunoaffinity, Immunoprecipitation.

Complete kit containing:
- prepackaged tubes with sufficient magnetic beads (4mg/tube) 
- Binding Buffer
- Washing Buffer
- Elution Buffers

Product capacity:
15 purifications (each from 0.6ml serum/ascite/cell supernatant) / Code Number 04400
30 purifications (each from 0.6ml serum/ascite/cell supernatant) / Code Number 04401

References:

1- Molecular Therapy doi:10.1038/sj.mt.6300289, Interactions Between Human Plasma Components and A Xenogenic Adenovirus Vector: Reduced Immunogenicity During Gene Transfer, Matthieu Perreau , Marie-Christine Guérin Christian Drouet, and Eric J Kremer

2-Biochimica and Biophysica Acta 173 (2008) 223462240 : Membrane porgestin receptor  alpha and  gamma renal epithelium, Julie Lemale, May Block-faure, Adrin Grimont, Boutaina El Abida, Martine Imbert-Teboul, Gilles Crambert